
Proceedings Paper
Full pupil line-scanning confocal microscope for imaging weakly scattering tissues: comparison to divided pupilFormat | Member Price | Non-Member Price |
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Paper Abstract
Confocal reflectance full-pupil and divided-pupil line-scanning microscopes
provide optical sectioning and image nuclear detail in skin.
Line-scanning with linear
detectors is a simpler alternative to point-scanning for imaging weakly scattering
epidermis and the oral epithelium. With illumination of 830 nm, a water immersion lens
of numerical aperture 0.9 and slit width three times smaller than the diffraction-limited
line width, the instrumental full width at half maximum (FWHM) optical sectioning (linespread
function) for the full-pupil design is 1.4 +/- 0.07 μm, which degrades through fullthickness
human epidermis to 2.8 +/- 0.78 μm. The lateral resolution is 0.7±0.10 μm,
which degrades to 1.6±0.28 μm through human epidermis. The
divided-pupil design
demonstrates instrumental optical sectioning of 1.7 μm, which degrades to 7.6 μm
through human epidermis. The lateral resolution is 1.0 μm, which degrades to 1.7 μm.
Heavy scattering in the dermis decreases contrast. Images of skin
in-vivo show nuclear
detail as expected with the predicted and experimentally verified sectioning. However,
pixel crosstalk and speckle artifact degrade image quality in strongly scattering and
aberrating tissues. The sources of degradation (aberration and scattering) are
evaluated for the two design to assess the feasibility of these techniques for in vivo
imaging.
Paper Details
Date Published: 12 February 2009
PDF: 6 pages
Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 718207 (12 February 2009); doi: 10.1117/12.809450
Published in SPIE Proceedings Vol. 7182:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)
PDF: 6 pages
Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 718207 (12 February 2009); doi: 10.1117/12.809450
Show Author Affiliations
Daniel S. Gareau, Memorial Sloan-Kettering Cancer Ctr. (United States)
Sanjee Abeytunge, Memorial Sloan-Kettering Cancer Ctr. (United States)
Sanjee Abeytunge, Memorial Sloan-Kettering Cancer Ctr. (United States)
Milind Rajadhyaksha, Memorial Sloan-Kettering Cancer Ctr. (United States)
Published in SPIE Proceedings Vol. 7182:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)
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