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Proceedings Paper

Molecular analysis of arterial remodeling: a novel application of infrared imaging
Author(s): Brad C. Herman; Rishi Kundi; Dai Yamanouchi; K. Craig Kent; Bo Liu; Nancy Pleshko
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Paper Abstract

Arterial remodeling, i.e. changes in size and/or structure of arteries, plays an important role in vascular disease. Conflicting findings have been reported as to whether an abundance of collagen causes inward or outward remodeling, phenomena that result in either a smaller or larger lumen, respectively. We hypothesize that the amount, type and quality of collagen influence the remodeling response. Here, we create mechanical injury to the rat carotid artery using a balloon catheter, and this leads to inward remodeling. Treatment of the artery with Connective Tissue Growth Factor (CTGF) causes outward remodeling. We investigated the arterial composition in injured CTGF-treated and non-CTGF-treated and sham CTGF-treated and non-CTGF treated arteries 14 days post-injury (n = 7-8 per group) using infrared imaging. A Perkin Elmer Spotlight Spectrum 300 FT-IR microscope was used for data collection. Cross-sections of paraffinembedded arteries were scanned at 2 cm-1 spectral resolution with spatial resolution of 6.25 μm/pixel, and data analyzed using Malvern Instruments ISys 5.0. Post-injury, we found a nearly 50% reduction in the average 1338/AM2 area ratio (correlated to collagen helical integrity). The most dramatic change was a 600% increase in the 1660/1690 peak height ratio, which has previously been related to collagen crosslink maturity. In all cases, CTGF treatment resulted in the observed changes in peak parameters normalized back to control values. Overall, these preliminary studies demonstrate that infrared imaging can provide insight into the underlying molecular changes that contribute to arterial disease.

Paper Details

Date Published: 12 February 2009
PDF: 12 pages
Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 71821H (12 February 2009); doi: 10.1117/12.809425
Show Author Affiliations
Brad C. Herman, Weill Cornell Medical College (United States)
Rishi Kundi, Weill Cornell Medical College (United States)
Dai Yamanouchi, Univ. of Wisconsin (United States)
K. Craig Kent, Univ. of Wisconsin (United States)
Bo Liu, Weill Cornell Medical College (United States)
Univ. of Wisconsin (United States)
Nancy Pleshko, Exponent (United States)

Published in SPIE Proceedings Vol. 7182:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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