
Proceedings Paper
Hollow fiber: a biophotonic implant for live cellsFormat | Member Price | Non-Member Price |
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Paper Abstract
The technical objective of this study has been to design, build and validate biocompatible hollow fiber implants based on
fluorescence with integrated biophotonics components to enable in fiber kinetic cell based assays. A human
osteosarcoma in vitro cell model fiber system has been established with validation studies to determine in fiber cell
growth, cell cycle analysis and organization in normal and drug treated conditions. The rationale for implant
development have focused on developing benchmark concepts in standard monolayer tissue culture followed by the
development of in vitro hollow fiber designs; encompassing imaging with and without integrated biophotonics.
Furthermore the effect of introducing targetable biosensors into the encapsulated tumor implant such as quantum dots for
informing new detection readouts and possible implant designs have been evaluated. A preliminary micro/macro
imaging approach has been undertaken, that could provide a mean to track distinct morphological changes in cells
growing in a 3D matrix within the fiber which affect the light scattering properties of the implant. Parallel engineering
studies have showed the influence of the optical properties of the fiber polymer wall in all imaging modes. Taken all
together, we show the basic foundation and the opportunities for multi-modal imaging within an in vitro implant format.
Paper Details
Date Published: 12 February 2009
PDF: 11 pages
Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 71820V (12 February 2009); doi: 10.1117/12.809391
Published in SPIE Proceedings Vol. 7182:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)
PDF: 11 pages
Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 71820V (12 February 2009); doi: 10.1117/12.809391
Show Author Affiliations
Oscar F. Silvestre, Cardiff Univ. (United Kingdom)
Mark D. Holton, Swansea Univ. (United Kingdom)
Huw D. Summers, Swansea Univ. (United Kingdom)
Mark D. Holton, Swansea Univ. (United Kingdom)
Huw D. Summers, Swansea Univ. (United Kingdom)
Paul J. Smith, Cardiff Univ. (United Kingdom)
Rachel J. Errington, Cardiff Univ. (United Kingdom)
Rachel J. Errington, Cardiff Univ. (United Kingdom)
Published in SPIE Proceedings Vol. 7182:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)
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