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Proceedings Paper

Label-free imaging of arterial tissues using photonic crystal fiber (PCF) based nonlinear optical microscopic system
Author(s): Alex C. T. Ko; Andrew Ridsdale; Adrian F. Pegoraro; Michael S. D. Smith; Leila B. Mostaço-Guidolin; Mark D. Hewko; Elicia M. Kohlenberg; Bernie J. Schattka; Masashi Shiomi; Albert Stolow; Michael G. Sowa
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Paper Abstract

Nonlinear optical (NLO) microscopy provides a minimally invasive optical method for fast molecular imaging at subcellular resolution with 3D sectioning capability in thick, highly scattering biological tissues. In the current study, we demonstrate the imaging of arterial tissue using a nonlinear optical microscope based on photonic crystal fiber and a single femto-second oscillator operating at 800nm. This NLO microscope system is capable of simultaneous imaging extracellular elastin/collagen structures and lipid distribution within aortic tissue obtained from coronary atherosclerosis-prone WHHLMI rabbits (Watanabe heritable hyperlipidemic rabbit-myocardial infarction) Clear pathological differences in arterial lumen surface were observed between healthy arterial tissue and atherosclerotic lesions through NLO imaging.

Paper Details

Date Published: 23 February 2009
PDF: 8 pages
Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 718204 (23 February 2009); doi: 10.1117/12.809361
Show Author Affiliations
Alex C. T. Ko, National Research Council Canada (Canada)
Andrew Ridsdale, National Research Council Canada (Canada)
Adrian F. Pegoraro, National Research Council Canada (Canada)
Michael S. D. Smith, National Research Council Canada (Canada)
Leila B. Mostaço-Guidolin, National Research Council Canada (Canada)
Mark D. Hewko, National Research Council Canada (Canada)
Elicia M. Kohlenberg, National Research Council Canada (Canada)
Bernie J. Schattka, National Research Council Canada (Canada)
Masashi Shiomi, Kobe Univ School of Medicine (Japan)
Albert Stolow, National Research Council Canada (Canada)
Michael G. Sowa, National Research Council Canada (Canada)

Published in SPIE Proceedings Vol. 7182:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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