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Proceedings Paper

SHIM and TPEM: getting more information from non linear excitation
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Paper Abstract

Several endogenous protein structures give rise to second harmonic generation (SHG) - second order nonabsorbative energy doubling of an excitation laser line. The orientation of collagen fibers within tissues such as tendons or ligaments is of primary importance. In this study, we propose a method to map the orientation of collagen fibers of a tendon. The method uses only few images acquired at specific polarizations of the input laser beam by rotating the sample on the stage. This procedure is implemented both in backward and forward scattering pathways. The improved details should clarify the backscattered signal nature.

Paper Details

Date Published: 13 February 2009
PDF: 4 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 718335 (13 February 2009);
Show Author Affiliations
Paolo Bianchini, Univ. of Genoa (Italy)
Alberto Diaspro, Univ. of Genoa (Italy)
Italian Institute of Technology (Italy)
IBF, CNR (Italy)

Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)

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