Share Email Print

Proceedings Paper

Affinity fluorescence-labeled peptides for the early detection of cancer in Barrett's esophagus
Author(s): Meng Li; Shaoying Lu; Cyrus Piraka; Henry Appelman; Rich Kwon; Roy Soetikno; Tonya Kaltenbach; Thomas D. Wang
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Fluorescence-labeled peptides that affinity bind to neoplastic mucsosa are promising for use as a specific contrast agent in the detection of pre-malignant tissue in the esophagus. This method is can be used to identify expression of biological markers associated with dysplasia on endoscopic imaging as a guide for biopsy and represents a novel method for the early detection and prevention of cancer. We demonstrate the use of phage display to select affinity peptides and identify the sequence "ASYNYDA" that binds with high target-to-background ratio to dysplastic esophageal mucosa compared to that of intestinal metaplasia. Validation of preferential binding is demonstrated for neoplasia in the setting of Barrett's esophagus. An optimal tradeoff between sensitivity and specificity of 82% and 85% was found at the relative threshold of 0.60 with a target-to-background ratio of 1.81 and an area under the ROC curve of 0.87. Peptides are a novel class of ligand for targeted detection of pre-malignant mucosa for purposes of screening and surveillance.

Paper Details

Date Published: 2 March 2009
PDF: 9 pages
Proc. SPIE 7172, Endoscopic Microscopy IV, 71720J (2 March 2009); doi: 10.1117/12.809140
Show Author Affiliations
Meng Li, Univ. of Michigan (United States)
Shaoying Lu, Xi'an Jiaotong Univ. (China)
Cyrus Piraka, Univ. of Michigan (United States)
Henry Appelman, Univ. of Michigan (United States)
Rich Kwon, Univ. of Michigan (United States)
Roy Soetikno, VA Palo Alto Health Care System (United States)
Tonya Kaltenbach, VA Palo Alto Health Care System (United States)
Thomas D. Wang, Univ. of Michigan (United States)
VA Palo Alto Health Care System (United States)
VA Ann Arbor Health Care System (United States)

Published in SPIE Proceedings Vol. 7172:
Endoscopic Microscopy IV
Guillermo J. Tearney; Thomas D. Wang, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?