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Proceedings Paper

SPDM: single molecule superresolution of cellular nanostructures
Author(s): Rainer Kaufmann; Paul Lemmer; Manuel Gunkel; Yanina Weiland; Patrick Müller; Michael Hausmann; David Baddeley; Roman Amberger; Christoph Cremer
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Paper Abstract

Novel methods of visible light microscopy have overcome the limits of resolution hitherto thought to be insurmountable. The localization microscopy technique presented here based on the principles of Spectral Precision Distance Microscopy (SPDM) with conventional fluorophores under special physical conditions allows to measure the spatial distribution of single fluorescence labeled molecules in entire cells with macromolecular precision which is comparable to a macromolecular effective optical resolution. Based on detection of single molecules, in a novel combination of SPDM and Spatially Modulated Illumination (SMI) microscopy, a lateral (2D) effective optical resolution of cellular nanostructures around 10 - 20 nm (about 1/50th of the exciting wavelength) and a three dimensional (3D) effective optical resolution in the range of 40 - 50 nm are achieved.

Paper Details

Date Published: 24 February 2009
PDF: 19 pages
Proc. SPIE 7185, Single Molecule Spectroscopy and Imaging II, 71850J (24 February 2009); doi: 10.1117/12.809109
Show Author Affiliations
Rainer Kaufmann, Univ. of Heidelberg (Germany)
Paul Lemmer, Univ. of Heidelberg (Germany)
Manuel Gunkel, Univ. of Heidelberg (Germany)
Yanina Weiland, Univ. of Heidelberg (Germany)
Patrick Müller, Univ. of Heidelberg (Germany)
Michael Hausmann, Univ. of Heidelberg (Germany)
David Baddeley, Univ. of Heidelberg (Germany)
Univ. of Auckland (New Zealand)
Roman Amberger, Univ. of Heidelberg (Germany)
Christoph Cremer, Univ. of Heidelberg (Germany)
Institute for Molecular Biophysics (United States)

Published in SPIE Proceedings Vol. 7185:
Single Molecule Spectroscopy and Imaging II
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann, Editor(s)

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