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Proceedings Paper

Detection of disseminated peritoneal tumors by fluorescein diacrylate in mice
Author(s): Yoshinori Harada; Hirokazu Furuta; Yasutoshi Murayama; Ping Dai; Yuta Fujikawa; Yasuteru Urano; Tetsuo Nagano; Koki Morishita; Akira Hasegawa; Tetsuro Takamatsu
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Paper Abstract

Tumor invasion to the peritoneum is a poor prognostic factor in cancer patients. Accurate diagnosis of disseminated peritoneal tumors is essential to accurate cancer staging. To date, peritoneal washing cytology during laparotomy has been used for diagnosis of peritoneal dissemination of gastrointestinal cancer, but its sensitivity has not been satisfactory. Thus, a more direct approach is indispensable to detect peritoneal dissemination in vivo. Fluorescein diacrylate (FDAcr) is an esterase-sensitive fluorescent probe derived from fluorescein. In cancer cells, fluorescent fluorescein generated by exogenous application of FDAcr selectively deposits owing to its stronger hydrolytic enzyme activity and its lower leakage rate. We examined whether FDAcr can specifically detect disseminated peritoneal tumors in athymic nude mouse models. Intraperitoneally administered FDAcr revealed disseminated peritoneal microscopic tumors not readily recognized on white-light imaging. These results suggest that FDAcr is a useful probe for detecting disseminated peritoneal tumors.

Paper Details

Date Published: 20 February 2009
PDF: 5 pages
Proc. SPIE 7190, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications, 719019 (20 February 2009); doi: 10.1117/12.809063
Show Author Affiliations
Yoshinori Harada, Kyoto Prefectural Univ. of Medicine (Japan)
Hirokazu Furuta, Kyoto Prefectural Univ. of Medicine (Japan)
Yasutoshi Murayama, Kyoto Prefectural Univ. of Medicine (Japan)
Ping Dai, Kyoto Prefectural Univ. of Medicine (Japan)
Yuta Fujikawa, The Univ. of Tokyo (Japan)
Yasuteru Urano, The Univ. of Tokyo (Japan)
Tetsuo Nagano, The Univ. of Tokyo (Japan)
Koki Morishita, Olympus Corp. (Japan)
Akira Hasegawa, Olympus Corp. (Japan)
Tetsuro Takamatsu, Kyoto Prefectural Univ. of Medicine (Japan)

Published in SPIE Proceedings Vol. 7190:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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