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Proceedings Paper

Dynamic molecular imaging of living cells by using Raman scattering
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Paper Abstract

Raman spectroscopy has been utilized to investigate properties of biomolecules due to its capability of detecting molecular vibrations that represent molecular species, structures and environmental conditions. In this research, we used Raman scattering to image dynamics of molecules in living cells. By combining the 532 nm excitation and a slitscanning detection technique, we observed dynamics of molecular distributions of cytochrome, protein beta sheets, and lipids in an unstained HeLa cell during mitosis with a frame rate of 5 minutes.

Paper Details

Date Published: 23 February 2009
PDF: 5 pages
Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 71820I (23 February 2009); doi: 10.1117/12.809053
Show Author Affiliations
Katsumasa Fujita, Osaka Univ. (Japan)
Keisaku Hamada, Osaka Univ. (Japan)
Nicholas Isaac Smith, Osaka Univ. (Japan)
Yasushi Inouye, Osaka Univ. (Japan)
Satoshi Kawata, Osaka Univ. (Japan)
The Institute of Physical and Chemical Research-RIKEN (Japan)

Published in SPIE Proceedings Vol. 7182:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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