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Proceedings Paper

Comparison of background corrected fluorescence correlation spectroscopy and fluorescence lifetime correlation spectroscopy: dilution series revisited
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Paper Abstract

We performed quantitative tests in order to compare the practical limits of FCS and FLCS. Unlike conventional FCS, FLCS yields precise and correct concentration values from as low as picomolar to micromolar concentrations. We discuss some of the inherent technical limitations of FCS and demonstrate that they are easily overcome by FLCS employing the simplest confocal detection scheme.

Paper Details

Date Published: 24 February 2009
PDF: 6 pages
Proc. SPIE 7185, Single Molecule Spectroscopy and Imaging II, 718508 (24 February 2009); doi: 10.1117/12.808883
Show Author Affiliations
Steffen Rüttinger, Physikalisch-Technische Bundesanstalt (Germany)
Peter Kapusta, PicoQuant GmbH (Germany)
Matthias Patting, PicoQuant GmbH (Germany)
Michael Wahl, PicoQuant GmbH (Germany)
Rainer Macdonald, Physikalisch-Technische Bundesanstalt (Germany)

Published in SPIE Proceedings Vol. 7185:
Single Molecule Spectroscopy and Imaging II
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann, Editor(s)

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