This study demonstrates a novel phase-sensitive surface plasman resonance biosensor (PS-SPRB) which is able to convert the phase modulation into amplitude modulation analytically via a differential amplifier. PS-SPRB is able to measure biomolecule interactions at ultra-low concentration relying on the properties of phase sensitive detection at shot-noise detection in a real-time. The common-phase noise-rejection mode is provided in PS-SPRB to be able to immune the background phase noise efficiently. The experimental results confirm the detection sensitivity of 0.00001 wt % concentration of sucrose solution and 10 fg/ml mouse IgG interaction with anti IgG in real time.

Date Published: 20 February 2009
PDF: 7 pages
Proc. SPIE 7186, Optical Diagnostics and Sensing IX, 71860I (20 February 2009); doi: 10.1117/12.808823

Published in SPIE Proceedings Vol. 7186:
Optical Diagnostics and Sensing IX
Gerard L. Coté, Editor(s)