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Proceedings Paper

Accelerated adhesion of grafted skins by laser-induced stress wave-based gene transfer of hepatocyte growth factor
Author(s): Kazuya Aizawa; Shunichi Sato; Daizoh Saitoh; Hitoshi Tsuda; Hiroshi Ashida; Minoru Obara
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Paper Abstract

In our previous study, we delivered plasmid DNA coding for human hepatocyto growth factor (hHGF) to rat skin grafts based on laser-induced stress wave (LISW), by which production of CD31-positive cells in the grafted skins was found to be enhanced, suggesting improved angiogenesis. In this study, we validated the efficacy of this method to accelerate adhesion of grafted skins; reperfusion and reepithelialization in the grafted skins were examined. As a graft, dorsal skin of a rat was exsected and its subcutaneous fat was removed. Plasmid DNA expression vector for hHGF was injected into the graft; on its back surface a laser target with a transparent sheet for plasma confinement was placed, and irradiated with three nanosecond laser pulses at a laser fluence of 1.2 J/cm2 (532 nm; spot diameter, 3 mm) to generate LISWs. After the application of LISWs, the graft was transplanted onto its donor site. We evaluated blood flow by laser Doppler imaging and analyzed reepithelialization based on immunohistochemistry as a function of postgrafting time. It was found that both reperfusion and reepithelialization were significantly enhanced for the grafts with gene transfection than for normal grafts; reepithelialization was completed within 7 days after transplantation with the transfected grafts. These findings demonstrate that adhesion of grafted skins can be accelerated by delivering HGF gene to the grafts based on LISWs.

Paper Details

Date Published: 19 February 2009
PDF: 10 pages
Proc. SPIE 7161, Photonic Therapeutics and Diagnostics V, 71610L (19 February 2009); doi: 10.1117/12.808663
Show Author Affiliations
Kazuya Aizawa, Keio Univ. (Japan)
Shunichi Sato, National Defense Medical College Research Institute (Japan)
Daizoh Saitoh, National Defense Medical College Research Institute (Japan)
Hitoshi Tsuda, National Defense Medical College (Japan)
Hiroshi Ashida, National Defense Medical College Research Institute (Japan)
Minoru Obara, Keio Univ. (Japan)

Published in SPIE Proceedings Vol. 7161:
Photonic Therapeutics and Diagnostics V
Henry Hirschberg M.D.; Brian Jet-Fei Wong M.D.; Kenton W. Gregory M.D.; Reza S. Malek; Nikiforos Kollias; Bernard Choi; Guillermo J. Tearney; Justus F. R. Ilgner; Steen J. Madsen; Laura Marcu; Haishan Zeng, Editor(s)

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