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Proceedings Paper

Fiber optic FTIR instrument for in vivo detection of colonic neoplasia
Author(s): Matthew Van Nortwick; John Hargrove; Rolf Wolters; James M. Crawford M.D.; May Arroyo; Mark Mackanos; Christopher H. Contag; Thomas D. Wang
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Paper Abstract

We demonstrate the proof of concept for use of a fiber optic FTIR instrument to perform in vivo detection of colonic neoplasia as an adjunct to medical endoscopy. FTIR is sensitive to the molecular composition of tissue, and can be used as a guide for biopsy by identifying pre-malignant tissue (dysplasia). First, we demonstrate the use of a silver halide optical fiber to collect mid-infrared absorption spectra in the 950 to 1800 cm-1 regime with high signal-to-noise from biopsy specimens of colonic mucosa tissue ex vivo. We observed subtle differences in wavenumber and magnitude of the absorbance peaks over this regime. We then show that optimal sub-ranges can be defined within this spectral regime and that spectral pre-processing can be performed to classify the tissue as normal, hyperplasia, or dysplasia with high levels of performance. We used a partial least squares discriminant analysis and a leave-one-subject-out crossvalidation strategy to classify the spectra. The results were compared with histology, and the optimal thresholds resulted in an overall sensitivity, specificity, accuracy, and positive predictive value of 96%, 92%, 93%, and 82%, respectively for this technique. We demonstrate that mid-infrared absorption spectra can be collected remotely with an optical fiber and used to identify colonic dysplasia with high accuracy. We are now developing an endoscope compatible optical fiber to use this technique clinically for the early detection of cancer.

Paper Details

Date Published: 2 March 2009
PDF: 8 pages
Proc. SPIE 7172, Endoscopic Microscopy IV, 71720K (2 March 2009); doi: 10.1117/12.808570
Show Author Affiliations
Matthew Van Nortwick, Univ. of Michigan (United States)
John Hargrove, STI Medical Systems (United States)
Rolf Wolters, STI Medical Systems (United States)
James M. Crawford M.D., Univ. of Florida College of Medicine (United States)
May Arroyo, Univ. of Florida College of Medicine (United States)
Mark Mackanos, Stanford Univ. (United States)
Christopher H. Contag, Stanford Univ. (United States)
Thomas D. Wang, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 7172:
Endoscopic Microscopy IV
Guillermo J. Tearney; Thomas D. Wang, Editor(s)

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