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Proceedings Paper

Label-free biosensing using a photonic crystal structure in a total-internal-reflection geometry
Author(s): Yunbo Guo; Jing Yong Ye; Charles Divin; Thommey P. Thomas; Andrzej Myc; Tommaso F. Bersano-Begey; James R. Baker Jr.; Theodore B. Norris
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Paper Abstract

A novel optical biosensor using a one-dimensional photonic crystal structure in a total-internal-reflection geometry (PCTIR) is presented and investigated for label-free biosensing applications. This simple configuration forms a micro Fabry- Perot resonator in the top layer which provides a narrow optical resonance to enable label-free, highly sensitive measurements for the presence of analytes on the sensing surface or the refractive index change of the surrounding medium in the enhanced evanescent field; and at the same time it employs an open sensing surface for real-time biomolecular binding detection. The high sensitivity of the sensor was experimentally demonstrated by bulk solvent refractive index changes, ultrathin molecular films adsorbed on the sensing surface, and real-time analytes binding, measuring both the spectral shift of the photonic crystal resonance and the change of the intensity ratio in a differential reflectance measurement. Detection limits of 7×10-8 RIU for bulk solvent refractive index, 6×10-5 nm for molecular layer thickness and 24 fg/mm2 for mass density were obtained, which represent a significant improvement relative to state-ofthe- art surface-plasmon-resonance (SPR)-based systems. The PC-TIR sensor is thus seen to be a promising technology platform for high sensitivity and accurate biomolecular detection.

Paper Details

Date Published: 16 February 2009
PDF: 12 pages
Proc. SPIE 7188, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VI, 71880B (16 February 2009); doi: 10.1117/12.808369
Show Author Affiliations
Yunbo Guo, Univ. of Michigan (United States)
Jing Yong Ye, Univ. of Michigan (United States)
Charles Divin, Univ. of Michigan (United States)
Thommey P. Thomas, Univ. of Michigan (United States)
Andrzej Myc, Univ. of Michigan (United States)
Tommaso F. Bersano-Begey, Univ. of Michigan (United States)
James R. Baker Jr., Univ. of Michigan (United States)
Theodore B. Norris, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 7188:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VI
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)

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