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Proceedings Paper

Biophysical interpretation and ex-vivo characterization of scattered light from tumor-associated breast stroma
Author(s): Ashley Laughney; Venkat Krishnaswamy; Mary Schwab; Wendy A. Wells; Keith D. Paulsen; Brian W. Pogue
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Paper Abstract

The purpose of this study was to extract scatter parameters related to tissue ultra-structures from freshly excised breast tissue and to assess whether evident changes in scatter across diagnostic categories is primarily influenced by variation in the composition of each tissues subtypes or by physical remodeling of the extra-cellular environment. Pathologists easily distinguish between epithelium, stroma and adipose tissues, so this classification was adopted for macroscopic subtype classification. Micro-sampling reflectance spectroscopy was used to characterize single-backscattered photons from fresh, excised tumors and normal reduction specimens with sub-millimeter resolution. Phase contrast microscopy (sub-micron resolution) was used to characterize forward-scattered light through frozen tissue from the DHMC Tissue Bank, representing normal, benign and malignant breast tissue, sectioned at 10 microns. The packing density and orientation of collagen fibers in the extracellular matrix (ECM) associated with invasive, normal and benign epithelium was evaluated using transmission electron microscopy (TEM). Regions of interest (ROIs) in the H&E stained tissues were identified for analysis, as outlined by a pathologist as the gold standard. We conclude that the scatter parameters associated with tumor specimens (Npatients=6, Nspecimens=13) significantly differs from that of normal reductions (Npatients=6, Nspecimens=10). Further, tissue subtypes may be identified by their scatter spectra at sub-micron resolution. Stromal tissue scatters significantly more than the epithelial cells embedded in its ECM and adipose tissue scatters much less. However, the scatter signature of the stroma at the sub-micron level is not particularly differentiating in terms of a diagnosis.

Paper Details

Date Published: 12 February 2009
PDF: 9 pages
Proc. SPIE 7174, Optical Tomography and Spectroscopy of Tissue VIII, 71741Y (12 February 2009); doi: 10.1117/12.808323
Show Author Affiliations
Ashley Laughney, Dartmouth College (United States)
Venkat Krishnaswamy, Dartmouth College (United States)
Mary Schwab, Dartmouth Hitchcock Medical Ctr. (United States)
Wendy A. Wells, Dartmouth Hitchcock Medical Ctr. (United States)
Keith D. Paulsen, Dartmouth College (United States)
Brian W. Pogue, Dartmouth College (United States)

Published in SPIE Proceedings Vol. 7174:
Optical Tomography and Spectroscopy of Tissue VIII
Bruce J. Tromberg; Arjun G. Yodh; Mamoru Tamura; Eva M. Sevick-Muraca; Robert R. Alfano, Editor(s)

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