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Proceedings Paper

Microflow cytometer
Author(s): Frances S. Ligler; Jeffrey S. Erickson; Joel P. Golden; Jason S. Kim; Mansoor Nasir; Peter J. Howell Jr.; Abel L. Thangawng; Lisa Hilliard; George P. Anderson
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Paper Abstract

A multi-analyte diagnostic system based on a novel microflow cytometer is under development as a portable, fielddeployable sensor for environmental monitoring and for rapid point-of-care and on-site diagnosis of exposure to biothreat agents. The technology relies on a unique method for ensheathing a sample stream in continuous flow past an illuminated interrogation region. This sheathing approach efficiently focuses particles in the interrogation region of the fluidic channel and minimizes clogging by complex samples. Fluorescently coded microspheres provide the capability for highly multiplexed assays. In this report, separation of six microsphere sets was demonstrated with determination of immunoassays on three of the six sets; comparison to the commercial platform was made.

Paper Details

Date Published: 19 February 2009
PDF: 5 pages
Proc. SPIE 7167, Frontiers in Pathogen Detection: From Nanosensors to Systems, 71670N (19 February 2009); doi: 10.1117/12.807671
Show Author Affiliations
Frances S. Ligler, Naval Research Lab. (United States)
Jeffrey S. Erickson, Naval Research Lab. (United States)
Joel P. Golden, Naval Research Lab. (United States)
Jason S. Kim, Naval Research Lab. (United States)
Mansoor Nasir, Naval Research Lab. (United States)
Peter J. Howell Jr., Naval Research Lab. (United States)
Abel L. Thangawng, Naval Research Lab. (United States)
Lisa Hilliard, Naval Research Lab. (United States)
George P. Anderson, Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 7167:
Frontiers in Pathogen Detection: From Nanosensors to Systems
Philippe M. Fauchet, Editor(s)

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