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Proceedings Paper

Mirror-based broadband scanner with minimized aberration
Author(s): Jiun-Yann Yu; Yu-Yi Tzeng; Chen-Han Huang; Hsiang-Chen Chui; Shi-Wei Chu
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Paper Abstract

To obtain specific biochemical information in optical scanning microscopy, labeling technique is routinely required. Instead of the complex and invasive sample preparation procedures, incorporating spectral acquisition, which commonly requires a broadband light source, provides another mechanism to enhance molecular contrast. But most current optical scanning system is lens-based and thus the spectral bandwidth is limited to several hundred nanometers due to anti-reflection coating and chromatic aberration. The spectral range of interest in biological research covers ultraviolet to infrared. For example, the absorption peak of water falls around 3 μm, while most proteins exhibit absorption in the UV-visible regime. For imaging purpose, the transmission window of skin and cerebral tissues fall around 1300 and 1800 nm, respectively. Therefore, to extend the spectral bandwidth of an optical scanning system from visible to mid-infrared, we propose a system composed of metallic coated mirrors. A common issue in such a mirror-based system is aberrations induced by oblique incidence. We propose to compensate astigmatism by exchanging the sagittal and tangential planes of the converging spherical mirrors in the scanning system. With the aid of an optical design software, we build a diffraction-limited broadband scanning system with wavefront flatness better than λ/4 at focal plane. Combined with a mirror-based objective this microscopic system will exhibit full spectral capability and will be useful in microscopic imaging and therapeutic applications.

Paper Details

Date Published: 24 February 2009
PDF: 8 pages
Proc. SPIE 7185, Single Molecule Spectroscopy and Imaging II, 718518 (24 February 2009); doi: 10.1117/12.807634
Show Author Affiliations
Jiun-Yann Yu, National Taiwan Univ. (Taiwan)
Yu-Yi Tzeng, National Taiwan Univ. (Taiwan)
Chen-Han Huang, National Taiwan Univ. (Taiwan)
National Cheng Kung Univ. (Taiwan)
Hsiang-Chen Chui, National Cheng Kung Univ. (Taiwan)
Shi-Wei Chu, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 7185:
Single Molecule Spectroscopy and Imaging II
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann, Editor(s)

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