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Proceedings Paper

Evaluation of membrane stacking in electron microscope images
Author(s): Gilles Hermann; Argyro Karathanou; Jean-Luc Buessler; Jean-Philippe Urban
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Paper Abstract

The objective of our work is to develop a tool for automatic analysis of 2D membrane protein crystal images in Transmission Electron Microscopy (TEM). The success of crystallization experiments is evaluated at high magnification. The crystalline structure of a membrane can be observed when no other membranes are superposed. It is therefore necessary to identify mono-layer membranes. In this paper we introduce an algorithm that determines the stacking-level of membranes. Our method determines a quantum, a gray-level quantity that is characteristic of a non-stacked membrane. In this way we are able to label each region qualitatively and construct a stacking-level map that distinguishes from non-stacked to up to four-level stacked membranes. This map provides the regions that will trigger a new image acquisition at higher magnification.

Paper Details

Date Published: 2 February 2009
PDF: 11 pages
Proc. SPIE 7251, Image Processing: Machine Vision Applications II, 725110 (2 February 2009); doi: 10.1117/12.807268
Show Author Affiliations
Gilles Hermann, Univ. de Haute Alsace (France)
Argyro Karathanou, Univ. de Haute Alsace (France)
Jean-Luc Buessler, Univ. de Haute Alsace (France)
Jean-Philippe Urban, Univ. de Haute Alsace (France)

Published in SPIE Proceedings Vol. 7251:
Image Processing: Machine Vision Applications II
Kurt S. Niel; David Fofi, Editor(s)

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