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Proceedings Paper

Phosphorescent nanoparticles and their applications for time-resolved luminescent biological assays
Author(s): Xuedong Song; Lei Huang; Mike Knotts; Bin Wu
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Paper Abstract

A new class of phosphorescent nanoparticles has been developed that use halogen-containing polymers and copolymers to encapsulate phosphorescent molecules. Their strong phosphorescence of long lifetime and large Stoke shift are not subject to oxygen quenching under ambient conditions due to the low oxygen permeability of the encapsulation matrix. The cross-linked phosphorescent particles are very stable and easily re-suspendable in aqueous media with surface functional groups to allow covalent tagging of biological recognition molecules such as antibodies. The conjugates can be used to provide very sensitive detection of analytes through time-resolved phosphorescence measurements. In addition to their applications for solution-based biological assays, those particles have also been demonstrated to be very useful for dry-chemistry-based time-resolved luminescent lateral flow assays.

Paper Details

Date Published: 20 February 2009
PDF: 7 pages
Proc. SPIE 7190, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications, 71900Q (20 February 2009); doi: 10.1117/12.806846
Show Author Affiliations
Xuedong Song, Kimberly Clark Corp. (United States)
Lei Huang, Kimberly Clark Corp. (United States)
Mike Knotts, Georgia Tech Research Institute (United States)
Bin Wu, Phosphorex, Inc. (United States)

Published in SPIE Proceedings Vol. 7190:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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