This paper examines the necessary technologies to be mastered in order to build a practical micro array-based immunoassay cassette and its processing station for protein analysis. The interdependence of surface-chemistry, dye stability and imaging are outlined showning why a treated 100 nm film of Nitrocellulose adhered by an intervening layer to glass offers an efficacious surface for immobilizing an array of protein probes. The properties of a storage surface to support in desiccated form, fluidize and transport additional reagents are outlined and a practical solution proposed. Wet and Dry imaging are compared. The steps and functions expected for an assay platform comprising processing station and biochip cassette are identified. The performance of a successful bench-top automated multiplex immunoassay system is briefly described

Date Published: 23 February 2009
PDF: 9 pages
Proc. SPIE 7207, Microfluidics, BioMEMS, and Medical Microsystems VII, 72070F (23 February 2009); doi: 10.1117/12.806639

Published in SPIE Proceedings Vol. 7207:
Microfluidics, BioMEMS, and Medical Microsystems VII
Wanjun Wang, Editor(s)