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Proceedings Paper

Optical cell monitoring system for underwater targets
Author(s): SangJun Moon; Fahim Manzur; Tariq Manzur; Utkan Demirci
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Paper Abstract

We demonstrate a cell based detection system that could be used for monitoring an underwater target volume and environment using a microfluidic chip and charge-coupled-device (CCD). This technique allows us to capture specific cells and enumerate these cells on a large area on a microchip. The microfluidic chip and a lens-less imaging platform were then merged to monitor cell populations and morphologies as a system that may find use in distributed sensor networks. The chip, featuring surface chemistry and automatic cell imaging, was fabricated from a cover glass slide, double sided adhesive film and a transparent Polymethlymetacrylate (PMMA) slab. The optically clear chip allows detecting cells with a CCD sensor. These chips were fabricated with a laser cutter without the use of photolithography. We utilized CD4+ cells that are captured on the floor of a microfluidic chip due to the ability to address specific target cells using antibody-antigen binding. Captured CD4+ cells were imaged with a fluorescence microscope to verify the chip specificity and efficiency. We achieved 70.2 ± 6.5% capturing efficiency and 88.8 ± 5.4% specificity for CD4+ T lymphocytes (n = 9 devices). Bright field images of the captured cells in the 24 mm × 4 mm × 50 μm microfluidic chip were obtained with the CCD sensor in one second. We achieved an inexpensive system that rapidly captures cells and images them using a lens-less CCD system. This microfluidic device can be modified for use in single cell detection utilizing a cheap light-emitting diode (LED) chip instead of a wide range CCD system.

Paper Details

Date Published: 16 October 2008
PDF: 12 pages
Proc. SPIE 7112, Unmanned/Unattended Sensors and Sensor Networks V, 71120Q (16 October 2008); doi: 10.1117/12.802761
Show Author Affiliations
SangJun Moon, Brigham and Women's Hospital, Harvard Medical School (United States)
Fahim Manzur, Brigham and Women's Hospital, Harvard Medical School (United States)
Tariq Manzur, Naval Undersea Warfare Ctr. (United States)
Utkan Demirci, Brigham and Women's Hospital, Harvard Medical School (United States)
Harvard-MIT Health Sciences and Technology (United States)

Published in SPIE Proceedings Vol. 7112:
Unmanned/Unattended Sensors and Sensor Networks V
Edward M. Carapezza, Editor(s)

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