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Proceedings Paper

A comparison of three different micro-tomography systems for accurate determination of microvascular parameters
Author(s): P. D. Lee; R. C. Atwood; P. Rockett; M. A. Konerding; J. R. Jones; C. A. Mitchell
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Paper Abstract

The investigation of micro-vessel dimensions in 3D is currently problematic due to their complex structures and fine scale. Quantification of vascular parameters is important in several fields of biomedicine; including embryogenesis, wound healing, diseases characterized by uncontrolled angiogenesis (e.g. tumor growth and metastasis) and the development of implantable bio-materials where a functional vascular supply is critical to their successful integration into host tissue. However, techniques that can resolve the micron-scaled features of these capillary beds, such as scanning electron and confocal microscopy, do not allow for total image reconstitution in 3D in thick tissue samples [1]. The present study describes the use of an in vivo corrosion casting technique that provides a stable replica of the microvascular network and the subsequent evaluation of three different μCT systems in order to accurately quantify vessel dimensions. Stable replicas of micro-vascular networks in neonatal mouse eyes were first created using in vivo vascular corrosion casting and then imaged using a unique, laboratory scale, μCT unit. This system combines a LaB6 cathode with high-performance electron optics to obtain a high resolution x-ray source. Novel image analysis was then applied to the reconstructions to quantify the morphological parameters of the hyaloid vascular plexi in the developing eyes of post-natal day 2 (P2) wild-type mice. These results are compared to synchrotron scans, establishing vascular casting and x-ray μCT as a valid laboratory scale experimental method for accurate 3D quantification of the microvasculature, with potential applications to a wide variety of fields in biological and medical research.

Paper Details

Date Published: 15 September 2008
PDF: 9 pages
Proc. SPIE 7078, Developments in X-Ray Tomography VI, 70780E (15 September 2008); doi: 10.1117/12.795558
Show Author Affiliations
P. D. Lee, Imperial College London (United Kingdom)
R. C. Atwood, Imperial College London (United Kingdom)
P. Rockett, Imperial College London (United Kingdom)
M. A. Konerding, Johannes Gutenberg Univ., Mainz (Germany)
J. R. Jones, Imperial College London (United Kingdom)
C. A. Mitchell, Univ. of Ulster (United Kingdom)

Published in SPIE Proceedings Vol. 7078:
Developments in X-Ray Tomography VI
Stuart R. Stock, Editor(s)

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