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Proceedings Paper

Automatable lipid bilayer formation for ion channel studies
Author(s): Jason L. Poulos; Hyunwoo Bang; Tae-Joon Jeon; Jacob J. Schmidt
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Paper Abstract

Transmembrane proteins and ion channels are important drug targets and have been explored as single molecule sensors. For these proteins to function normally they must be integrated within lipid bilayers; however, the labor and skill required to create artificial lipid bilayers have the limited the possible applications utilizing these proteins. In order to reduce the complexity and cost of lipid bilayer formation and measurement, we have modified a previously published lipid bilayer formation technique using mechanically contacted monolayers so that the process is automatable, requiring minimal operator input. Measurement electronics are integrated with the fluid handling system, greatly reducing the time and operator feedback characteristically required of traditional bilayer experiments. To demonstrate the biological functionality of the resultant bilayers and the system's capabilities as a membrane platform, the ion channel gramicidin A was incorporated and measured with this system.

Paper Details

Date Published: 29 August 2008
PDF: 6 pages
Proc. SPIE 7035, Biosensing, 703509 (29 August 2008); doi: 10.1117/12.795375
Show Author Affiliations
Jason L. Poulos, Univ. of California, Los Angeles (United States)
Hyunwoo Bang, Univ. of California, Los Angeles (United States)
Tae-Joon Jeon, Univ. of California, Los Angeles (United States)
Jacob J. Schmidt, Univ. of California, Los Angeles (United States)

Published in SPIE Proceedings Vol. 7035:
Manijeh Razeghi; Hooman Mohseni, Editor(s)

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