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Proceedings Paper

Establishment of mesenchymal stem cells derived from bone marrow and synovium of transgenic rats expressing dual reporter genes
Author(s): Masafumi Horie; Ichiro Sekiya; Takeshi Muneta; Takashi Murakami; Eiji Kobayashi M.D.
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Paper Abstract

Mesenchymal stem cells (MSCs) are an attractive cell source for regenerative medicine because they can be harvested in a relatively less invasive manner, easily isolated, and expanded with multipotentiality. Bone marrow seems to be the most commonly used tissue as a source for MSCs at present. However, there are emerging reports to describe that MSCs exist in most mesenchymal tissues. We have recently compared in vivo chondrogenic potential in MSCs derived from various mesenchymal tissues and demonstrated that synovium-MSCs and bone marrow-MSCs possessed greater chondrogenic ability than other mesenchymal tissue-derived MSCs. This indicates that those MSCs are promising cellular sources for cartilage regeneration. As the fate of synovium-MSCs is unclear after transplantation, we herein established MSCs using double transgenic rats expressing either Luciferase/GFP or Luciferase/LacZ. The cellular fate of MSCs could be traced by an in vivo luciferase-based luminescent imaging system, and also followed histologically by green fluorescence and by X-gal staining, respectively. Thus, both synovium-MSCs and bone marrow-MSCs expressing Luciferase/GFP or Luciferase/LacZ provide powerful tools not only for cell tracking in vivo but also for histological analysis, leading to a compelling experimental model of cartilage regeneration with cell therapy.

Paper Details

Date Published: 13 February 2008
PDF: 8 pages
Proc. SPIE 6868, Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes, 68680V (13 February 2008); doi: 10.1117/12.791432
Show Author Affiliations
Masafumi Horie, Tokyo Medical and Dental Univ. (Japan)
Ichiro Sekiya, Tokyo Medical and Dental Univ. (Japan)
Takeshi Muneta, Tokyo Medical and Dental Univ. (Japan)
Takashi Murakami, Ctr. for Molecular Medicine, Jichi Medical Univ. (Japan)
Eiji Kobayashi M.D., Ctr. for Molecular Medicine, Jichi Medical Univ. (Japan)

Published in SPIE Proceedings Vol. 6868:
Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes
Alexander P. Savitsky; Robert E. Campbell; Robert M. Hoffman, Editor(s)

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