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Proceedings Paper

Insight into the effect of Bim in Photofrin-PDT-induced apoptosis
Author(s): Xianwang Wang; Da Xing; Lei Liu; Jiaxing Song
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Paper Abstract

Photodynamic therapy (PDT) induces cell apoptosis mainly by the mitochondrial pathway. The Bcl-2 family proteins play an important role in this pathway. However, the detailed mechanism is not elucidated. The current study was aimed to determine whether Bim, a BH3-only protein of Bcl-2 family, was involved during Photofrin-PDT-induced apoptosis in ASTC-a-1 and MCF-7 cell lines. The data show that massive reactive oxygen species (ROS) were generated firstly after Photofrin-PDT treatment, leading to the disappearance of the mitochondrial membrane potential. Furthermore, the activation of Bim and Bax were dynamically observed. Confocal imaging of the cells transfected with GFP-BimL demonstrated that BimL translocate to mitochondria occurred about 12 min after PDT treatment. Bax activation occurred about 15 min after PDT treatment. These results suggest that Bim, as an effectors, was involved in Photofrin-PDT-induced apoptosis.

Paper Details

Date Published: 11 January 2008
PDF: 7 pages
Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 68261M (11 January 2008); doi: 10.1117/12.783077
Show Author Affiliations
Xianwang Wang, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Lei Liu, South China Normal Univ. (China)
Jiaxing Song, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 6826:
Optics in Health Care and Biomedical Optics III
Xingde Li; Qingming Luo; Ying Gu M.D., Editor(s)

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