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Proceedings Paper

DMS-prefiltered mass spectrometry for the detection of biomarkers
Author(s): Stephen L. Coy; Evgeny V. Krylov; Erkinjon G. Nazarov
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Paper Abstract

Technologies based on Differential Mobility Spectrometry (DMS) are ideally matched to rapid, sensitive, and selective detection of chemicals like biomarkers. Biomarkers linked to exposure to radiation, exposure to CWA's, exposure to toxic materials (TICs and TIMs) and to specific diseases are being examined in a number of laboratories. Screening for these types of exposure can be improved in accuracy and greatly speeded up by using DMS-MS instead of slower techniques like LC-MS and GC-MS. We have performed an extensive series of tests with nanospray-DMS-mass spectroscopy and standalone nanospray-DMS obtaining extensive information on chemistry and detectivity. DMS-MS systems implemented with low-resolution, low-cost, portable mass-spectrometry systems are very promising. Lowresolution mass spectrometry alone would be inadequate for the task, but with DMS pre-filtration to suppress interferences, can be quite effective, even for quantitative measurement. Bio-fluids and digests are well suited to ionization by electrospray and detection by mass-spectrometry, but signals from critical markers are overwhelmed by chemical noise from unrelated species, making essential quantitative analysis impossible. Sionex and collaborators have presented data using DMS to suppress chemical noise, allowing detection of cancer biomarkers in 10,000-fold excess of normal products1,2. In addition, a linear dynamic range of approximately 2,000 has been demonstrated with accurate quantitation3. We will review the range of possible applications and present new data on DMS-MS biomarker detection.

Paper Details

Date Published: 17 April 2008
PDF: 10 pages
Proc. SPIE 6954, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing IX, 695411 (17 April 2008); doi: 10.1117/12.782437
Show Author Affiliations
Stephen L. Coy, Sionex Corp. (United States)
Evgeny V. Krylov, Sionex Corp. (United States)
Erkinjon G. Nazarov, Sionex Corp. (United States)

Published in SPIE Proceedings Vol. 6954:
Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing IX
Augustus Way Fountain III; Patrick J. Gardner, Editor(s)

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