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Proceedings Paper

FDTD simulation of optical phase contrast microscope imaging
Author(s): Stoyan Tanev; James Pond; Paul Paddon; Valery V. Tuchin
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Paper Abstract

The Finite-Difference Time-Domain (FDTD) approach is applied to model optical phase contrast microscope (OPCM) imaging of Gold nanoparticles (NPs) in singe biological cells. We first demonstrate the effect of optical clearing on the optical phase contrast microscope image of a realistic size biological cell containing a cytoplasm, a nucleus and a membrane. The FDTD-based OPCM model is then applied to visualize the presence of a cluster of Gold NPs in the cytoplasm at optical conditions. To the best of our knowledge, this is the first study using the FDTD approach in combination with Fourier optics techniques to construct OPCM images of realistic size cells containing NPs. The results demonstrate the potential of the FDTD modelling approach and extend its area of applicability into a new biomedical research area.

Paper Details

Date Published: 2 May 2008
PDF: 9 pages
Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 69912D (2 May 2008); doi: 10.1117/12.781514
Show Author Affiliations
Stoyan Tanev, Carleton Univ. (Canada)
James Pond, Lumerical Solutions, Inc. (Canada)
Paul Paddon, Lumerical Solutions, Inc. (Canada)
Valery V. Tuchin, Saratov State Univ. (Russia)
Institute of Precise Mechanics and Control (Russia)

Published in SPIE Proceedings Vol. 6991:
Biophotonics: Photonic Solutions for Better Health Care
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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