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Proceedings Paper

Fourier transform spectral interferometric coherent anti-stokes Raman scattering (FTSI-CARS) microscopy
Author(s): Sang-Hyun Lim; Bi-Chang Chen; Jiha Sung
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Paper Abstract

A new multiplex CARS (coherent anti-Stokes Raman scattering) microscopy technique with a single ultrafast laser pulse is demonstrated. All the pump, Stokes, probe pulses are selected inside a single broadband cavity dumping Ti:Sapphire oscillator laser pulse. The measured CARS signal is a coherent sum of the resonant and non-resonant signals, leading to a complicated vibrational line shape due to the spectral interference. The resonant and non-resonant CARS signals, however, have different symmetries in the time domain due to the causality principle of the vibrationally resonant excitation. A new Fourier Transform Spectral interferometry (FTSI) is developed to extract the full complex quantity of the vibrationally resonant signal against the non-resonant one utilizing the different time symmetry. This method can generate Raman-like vibrational spectrum in a single experimental measurement, which can be readily applied to a vibrational hyperspectral imaging. Current sensitivity, available CARS window and application to hyperspectral vibrational microscopy are discussed.

Paper Details

Date Published: 15 February 2008
PDF: 8 pages
Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 686013 (15 February 2008); doi: 10.1117/12.781465
Show Author Affiliations
Sang-Hyun Lim, Univ. of Texas at Austin (United States)
Bi-Chang Chen, Univ. of Texas at Austin (United States)
Jiha Sung, Univ. of Texas at Austin (United States)

Published in SPIE Proceedings Vol. 6860:
Multiphoton Microscopy in the Biomedical Sciences VIII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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