
Proceedings Paper
Confirmatory measurement channels for LIF-based bioaerosol instrumentationFormat | Member Price | Non-Member Price |
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Paper Abstract
As part of the U.S. Department of Homeland Security Detect-to-Protect (DTP) program, a multilab [Sandia National
Laboratories (SNL), Lawrence Livermore National Laboratories (LLNL), Pacific Northwest National Laboratory
(PNNL), Oak Ridge National Laboratory (ORNL), and Los Alamos National Laboratory (LANL)] effort is addressing
the need for useable detect-to-warn bioaerosol sensors for public facility protection. Towards this end, the SNL team is
investigating the use of rapid fluorogenic staining to infer the protein content of bioaerosols. This is being implemented
in a flow cytometer wherein each particle detected generates coincident signals of correlated forward scatter, side
scatter, and fluorescence. Several thousand such coincident signal sets are typically collected to generate a distribution
describing the probability of observing a particle with certain scattering and fluorescence values. These data are
collected for sample particles in both a stained and unstained state. A linear unmixing analysis is performed to
differentiate components in the mixture. In this paper, we discuss the implementation of the staining process and the
cytometric measurement, the results of their application to the analysis of known and blind samples, and a potential
instrumental implementations that would use staining.
Paper Details
Date Published: 15 April 2008
PDF: 12 pages
Proc. SPIE 6945, Optics and Photonics in Global Homeland Security IV, 69450S (15 April 2008); doi: 10.1117/12.777698
Published in SPIE Proceedings Vol. 6945:
Optics and Photonics in Global Homeland Security IV
Craig S. Halvorson; Daniel Lehrfeld; Theodore T. Saito, Editor(s)
PDF: 12 pages
Proc. SPIE 6945, Optics and Photonics in Global Homeland Security IV, 69450S (15 April 2008); doi: 10.1117/12.777698
Show Author Affiliations
Scott E. Bisson, Sandia National Labs. (United States)
Robert W. Crocker, Sandia National Labs. (United States)
Thomas J. Kulp, Sandia National Labs. (United States)
Robert W. Crocker, Sandia National Labs. (United States)
Thomas J. Kulp, Sandia National Labs. (United States)
Thomas A. Reichardt, Sandia National Labs. (United States)
Peter T. A. Reilly, Oak Ridge National Lab. (United States)
William B. Whitten, Oak Ridge National Lab. (United States)
Peter T. A. Reilly, Oak Ridge National Lab. (United States)
William B. Whitten, Oak Ridge National Lab. (United States)
Published in SPIE Proceedings Vol. 6945:
Optics and Photonics in Global Homeland Security IV
Craig S. Halvorson; Daniel Lehrfeld; Theodore T. Saito, Editor(s)
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