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Proceedings Paper

A nanofluidic system for massively parallel PCR
Author(s): Colin Brenan; Tom Morrison; Douglas Roberts; James Hurley
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Paper Abstract

Massively parallel nanofluidic systems are lab-on-a-chip devices where solution phase biochemical and biological analyses are implemented in high density arrays of nanoliter holes micro-machined in a thin platen. Polymer coatings make the interior surfaces of the holes hydrophilic and the exterior surface of the platen hydrophobic for precise and accurate self-metered loading of liquids into each hole without cross-contamination. We have created a "nanoplate" based on this concept, equivalent in performance to standard microtiter plates, having 3072 thirty-three nanoliter holes in a stainless steel platen the dimensions of a microscope slide. We report on the performance of this device for PCR-based single nucleotide polymorphism (SNP) genotyping or quantitative measurement of gene expression by real-time PCR in applications ranging from plant and animal diagnostics, agricultural genetics and human disease research.

Paper Details

Date Published: 12 February 2008
PDF: 11 pages
Proc. SPIE 6886, Microfluidics, BioMEMS, and Medical Microsystems VI, 688609 (12 February 2008);
Show Author Affiliations
Colin Brenan, BioTrove, Inc. (United States)
Tom Morrison, BioTrove, Inc. (United States)
Douglas Roberts, BioTrove, Inc. (United States)
James Hurley, BioTrove, Inc. (United States)

Published in SPIE Proceedings Vol. 6886:
Microfluidics, BioMEMS, and Medical Microsystems VI
Wanjun Wang; Claude Vauchier, Editor(s)

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