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Proceedings Paper

In vivo photoacoustic imaging with multiple selective targeting using bioconjugated gold nanorods
Author(s): Chen-Wei Wei; Chao-Kang Liao; Ying-Yi Chen; Churng-Ren Chris Wang; Ann-Ann Ding; Dar-Bin Shiehd; Pai-Chi Li
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Paper Abstract

In this study, photoacoustic imaging is utilized to probe information from oncogene surface molecules of cancer cell with the aid of specific targeting. The ultimate goal is to provide prediction of clinical outcome and treatment response of anti-cancer drugs. Different from single targeting in most research, we accomplished multiple targeting to obtain a molecular profile potentially representing tumor characteristics or to locate the heterogeneous population in one lesion. By conjugating different antibodies to gold nanorods corresponding to different peak absorption bands, multiple targeting and simultaneous detection with photoacoustic imaging can be achieved with laser irradiation at the respective peak optical absorption wavelength. Her2 and EGFR were chosen as our primary target molecules. The targeting complex was evaluated in two types of oral cancer cells, OECM1 and Cal27. The OECM1 cell line overexpresses Her2 but has low expression of EGFR, while Cal27 cell line expresses both antibodies. Also, the targeting efficacy to OECM1 can be further improved by using mixed nanoprobes. The cancer cells were induced on the back of the mice by subcutaneous injection. The captured images show that both cancer cells exhibit a higher photoacoustic response (maximum 3 dB) than control groups with specific targeting, thus demonstrating the feasibility of multiple selective targeting with bioconjugated gold nanorods. Images of multiple targeting with mixed nanoprobes of OECM1 cells also reveal further enhancement of targeting (4 dB). The results showed potential of in vivo photoacoustic molecular imaging, providing a better guidance for diagnosis and treatment of cancer.

Paper Details

Date Published: 11 March 2008
PDF: 11 pages
Proc. SPIE 6856, Photons Plus Ultrasound: Imaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, 68560J (11 March 2008); doi: 10.1117/12.764454
Show Author Affiliations
Chen-Wei Wei, National Taiwan Univ. (Taiwan)
Chao-Kang Liao, National Taiwan Univ. (Taiwan)
Ying-Yi Chen, National Chung Cheng Univ. (Taiwan)
Churng-Ren Chris Wang, National Chung Cheng Univ. (Taiwan)
Ann-Ann Ding, National Cheng Kung Univ. (Taiwan)
Dar-Bin Shiehd, National Cheng Kung Univ. (Taiwan)
Pai-Chi Li, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6856:
Photons Plus Ultrasound: Imaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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