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Proceedings Paper

Two-photon absorption and transient photothermal imaging of pigments in tissues
Author(s): Tong Ye; Dan Fu; Thomas E. Matthews; Lian Hong; John D. Simon; Warren S. Warren
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Paper Abstract

As a main pigment in skin tissues, melanin plays an important role in photo-protecting skin from UV radiation. However, melanogenesis may be altered due to disease or environmental factors; for example, sun exposure may cause damage and mutation of melanocytes and induce melanoma. Imaging pigmentation changes may provide invaluable information to catch the malignant transformation in its early stage and in turn improve the prognosis of patients. We have demonstrated previously that transmission mode, two-photon, one- or two-color absorption microscopy could provide remarkable contrast in imaging melanin in skin. In this report we demonstrate significantly improved sensitivity, so that we are now able to image in epi-mode (or back reflection) in two-photon absorption. This improvement makes possible for us to characterize the different types of pigmentation on the skin in vivo at virtually any location. Another finding is that we can also image transient photothermal dynamics due to the light absorption of melanin. By carefully choosing excitation and probe wavelengths, we might be able to image melanin in different structures under different micro-environments in skin, which could provide useful photochemical and photophysical insights in understanding how pigments are involved in photoprotection and photodamage of cells.

Paper Details

Date Published: 8 February 2008
PDF: 10 pages
Proc. SPIE 6842, Photonic Therapeutics and Diagnostics IV, 684206 (8 February 2008); doi: 10.1117/12.764248
Show Author Affiliations
Tong Ye, Duke Univ. (United States)
Dan Fu, Princeton Univ. (United States)
Thomas E. Matthews, Duke Univ. (United States)
Lian Hong, Duke Univ. (United States)
John D. Simon, Duke Univ. (United States)
Warren S. Warren, Duke Univ. (United States)

Published in SPIE Proceedings Vol. 6842:
Photonic Therapeutics and Diagnostics IV
Kenton W. Gregory M.D.; Reza S. Malek M.D.; Nikiforos Kollias; Brian Jet-Fei Wong M.D.; Henry Hirschberg M.D.; Justus F. R. Ilgner M.D.; Steen J. Madsen; Guillermo J. Tearney M.D.; Bernard Choi; Haishan Zeng, Editor(s)

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