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Proceedings Paper

Label free high resolution in vivo optical imaging of microvessels
Author(s): Dan Fu; Thomas E. Matthews; Tong Ye; Warren S. Warren
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Paper Abstract

We have demonstrated a new optical microscopy technique for imaging microvasculature without any labeling. With a very sensitive two-color excited state absorption (ESA) measurement method, we demonstrated that oxy-hemoglobin and deoxy-hemoglobin show distinct excited state dynamics. Since this is a collinear measurement, we can readily apply it to the microscopic study of biological tissue. We have already demonstrated in vivo imaging of blood vessels in the nude mouse ear. Here we optimized the excitation and detection pulse train toward longer wavelengths, where tissue scatters less and greater penetration depth can be obtained. More importantly, we are able to separate arterioles from venules by employing different pump and probe wavelength combinations. This provides a powerful method to image blood vessels and their oxygenation level at the same time with micrometer resolution.

Paper Details

Date Published: 15 February 2008
PDF: 8 pages
Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68601Q (15 February 2008); doi: 10.1117/12.763969
Show Author Affiliations
Dan Fu, Princeton Univ. (United States)
Thomas E. Matthews, Duke Univ. (United States)
Tong Ye, Univ. of Alabama at Birmingham (United States)
Warren S. Warren, Duke Univ. (United States)

Published in SPIE Proceedings Vol. 6860:
Multiphoton Microscopy in the Biomedical Sciences VIII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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