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Proceedings Paper

Single molecules in fluorescence fluctuation spectroscopy: effective volume and photon counting histogram
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Paper Abstract

This paper presents a stochastic theory for the interpretation of photon counting histograms in fluorescence fluctuation spectroscopy (FFS). New concepts of an effective volume and a single molecule probability distribution are introduced to characterize a molecular species. Whereas the effective volume corresponds to the visibility of a molecular species in a given confocal setup, the single molecule probability distribution gives the signal measured for a single visible molecule. Specific properties of the effective volume and the single molecule probability distribution are discussed. Advantages arise for the high precision measurements of concentrations, mixtures, and binding constants especially for complex molecular environment, e.g. in flow systems and cell compartments.

Paper Details

Date Published: 21 February 2008
PDF: 12 pages
Proc. SPIE 6862, Single Molecule Spectroscopy and Imaging, 686204 (21 February 2008); doi: 10.1117/12.763904
Show Author Affiliations
Jörg Ackermann, FluIT Biosystems GmbH (Germany)
Harald Mathis, BioMOS, Fraunhofer Gesellschaft (Germany)
Benjamin Greiner, BioMOS, Fraunhofer Gesellschaft (Germany)

Published in SPIE Proceedings Vol. 6862:
Single Molecule Spectroscopy and Imaging
Jörg Enderlein; Zygmunt K. Gryczynski; Rainer Erdmann, Editor(s)

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