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Proceedings Paper

Flexible enclosure for fluidic sealing of microcomponents
Author(s): Takaya Ueda; Bonnie L. Gray; Yuchun Chen; Paul Li
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Paper Abstract

A flexible enclosure for fluidic sealing of SU-8 microcomponents was developed using polydimethylsiloxane (PDMS). The flexible enclosure can be mechanically assembled to SU-8 microchannels even in the presence of liquid. The seal is mechanical and completely reversible, thus allowing the channels to already contain immobilized enzymes or cells prior to assembly. To provide such a reversible fluid tight lid, the PDMS and SU-8 substrates contain interlocking structures that facilitate assembly and sealing together of the two substrates. Interlocking 1 mm ridges/holes and 400 μm ridges/grooves were fabricated on PDMS and SU-8 surfaces that hold the two pieces in place to prevent PDMS lid and SU-8 microchannels separation during handling and fluid flow. Furthermore, a 20 μm bump on the PDMS surface, which has the same width and length dimensions as the 400 μm deep SU-8 microchannel, acts as a plug to keep the fluid within the microchannel and prevent leakage between the PDMS-SU-8 interface. The assembled microchannels and enclosures can withstand manually applied fluidic pressure via a syringe which is noticeably higher than channels with a simple lid and no interconnect structures. The device was additionally quantified for pressure versus flow rate using a syringe pump and pressure sensor. The seal remained leak free up to 0.6 ml/min and 2.36 kPa. In addition, a preliminary cell viability test was conducted with leukemia cells and we observed that cells lived in the channel microenvironment for 24 hours.

Paper Details

Date Published: 12 February 2008
PDF: 9 pages
Proc. SPIE 6886, Microfluidics, BioMEMS, and Medical Microsystems VI, 68860P (12 February 2008); doi: 10.1117/12.763766
Show Author Affiliations
Takaya Ueda, Simon Fraser Univ. (Canada)
Bonnie L. Gray, Simon Fraser Univ. (Canada)
Yuchun Chen, Simon Fraser Univ. (Canada)
Paul Li, Simon Fraser Univ. (Canada)

Published in SPIE Proceedings Vol. 6886:
Microfluidics, BioMEMS, and Medical Microsystems VI
Wanjun Wang; Claude Vauchier, Editor(s)

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