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Proceedings Paper

Specific cellular delivery and intracellular fate of quantum dot-peptide and quantum dot-polymer nanoassemblies
Author(s): James B. Delehanty; Christopher E. Bradburne; Igor L. Medintz; Dorothy Farrell; Thomas Pons; Florence M. Brunel; Philip E. Dawson; Hedi Mattoussi
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Paper Abstract

Luminescent semiconductor quantum dots (QDs) possess several unique optical and spectroscopic properties that are of great interest and promise in biology. These properties suggest that QDs will be integral to the development of the next generation of biosensors capable of detecting molecular processes in both living and fixed cells. We are developing robust and facile delivery schemes for the selective intracellular delivery of QD-based nanoassemblies. These schemes are based upon the self-assembly and subsequent cellular uptake of QD-peptide and QD-polymer bioconjugates. The QD-peptide structures are generated by the self-assembly of the peptide onto CdSe-ZnS core-shell QDs via metal ion coordination between the peptide's polyhistidine motif and the Zn-rich QD shell. The polymer-based QD assemblies are formed via the electrostatic interaction of aqueous cationic liposomes with available carboxylate moieties on the QD surface ligands. Cellular delivery experiments utilizing both delivery schemes will be presented. The advantages and disadvantages of each approach will be discussed, including the intracellular fate and stability of the QD-nanoassemblies.

Paper Details

Date Published: 26 February 2008
PDF: 8 pages
Proc. SPIE 6866, Colloidal Quantum Dots for Biomedical Applications III, 68660K (26 February 2008); doi: 10.1117/12.763760
Show Author Affiliations
James B. Delehanty, Naval Research Lab. (United States)
Christopher E. Bradburne, Naval Research Lab. (United States)
Igor L. Medintz, Naval Research Lab. (United States)
Dorothy Farrell, Naval Research Lab. (United States)
Thomas Pons, Naval Research Lab. (United States)
Florence M. Brunel, The Scripps Research Institute (United States)
Philip E. Dawson, The Scripps Research Institute (United States)
Hedi Mattoussi, Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 6866:
Colloidal Quantum Dots for Biomedical Applications III
Marek Osinski; Thomas M. Jovin; Kenji Yamamoto, Editor(s)

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