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Proceedings Paper

Cytometric biochips with optically active surfaces for spatial engineering of fluorescence excitation
Author(s): Huw D. Summers; Rachel J. Errington; Paul Smith; Daniel R. Matthews; James F. Leary
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Paper Abstract

The development of surface-active biochips for control of fluorescence within microscopy platforms is described. These use surface-plasmon control to provide selective excitation of fluorescently labeled, live cell populations. These chips effectively combine a number of commonly used techniques such as SPR, TIRF and epi-fluorescence within a single device and have the potential to provide sub-cellular discrimination of excitation in 3-D. Thus within a single field of view we can selectively excite membrane versus cytoplasm and localise the excitation within the lateral plane to an area of a few square microns.

Paper Details

Date Published: 19 February 2008
PDF: 9 pages
Proc. SPIE 6886, Microfluidics, BioMEMS, and Medical Microsystems VI, 688603 (19 February 2008); doi: 10.1117/12.763645
Show Author Affiliations
Huw D. Summers, Cardiff Univ. (United Kingdom)
Rachel J. Errington, Cardiff Univ. (United Kingdom)
Paul Smith, Cardiff Univ. (United Kingdom)
Daniel R. Matthews, King's College London (United Kingdom)
James F. Leary, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 6886:
Microfluidics, BioMEMS, and Medical Microsystems VI
Wanjun Wang; Claude Vauchier, Editor(s)

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