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Proceedings Paper

Time-resolved luminescence measurements of the magnetic field effect on paramagnetic photosensitizers in photodynamic reactions
Author(s): O. Mermut; J.-P. Bouchard; J.-F. Cormier; P. Desroches; K. R. Diamond; M. Fortin; P. Gallant; S. Leclair; J.-S. Marois; I. Noiseux; J.-F. Morin; M. S. Patterson; M. Vernon
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Paper Abstract

The development of multimodal molecular probes and photosensitizing agents for use in photodynamic therapy (PDT) is vital for optimizing and monitoring cytotoxic responses. We propose a combinatorial approach utilizing photosensitizing molecules that are both paramagnetic and luminescent with multimodal functionality to perturb, control, and monitor molecular-scale reaction pathways in PDT. To this end, a time-domain single photon counting lifetime apparatus with a 400 nm excitation source has been developed and integrated with a variable low field magnet (0- 350mT). The luminescence lifetime decay function was measured in the presence of a sweeping magnetic field for a custom designed photosensitizing molecule in which photoinduced electron transfer was studied The photosensitizer studied was a donor-acceptor complex synthesized using a porphyrin linked to a fullerene molecule. The magneto-optic properties were investigated for the free-base photosensitizer complex as well as those containing either diamagnetic (paired electron) or paramagnetic (unpaired electron) metal centers, Zn(II) and Cu(II). The magnetic field was employed to affect and modify the spin states of radical pairs of the photosensitizing agents via magnetically induced hyperfine and Zeeman effects. Since the Type 1 reaction pathway of an excited triplet state photosensitizer involves the production of radical species, lifetime measurements were conducted at low dissolved oxygen concentration (0.01ppm) to elucidate the dependence of the magnetic perturbation on the photosensitization mechanistic pathway. To optimize the magnetic response, a solvent study was performed examining the dependence of the emission properties on the magnetic field in solutions of varying dielectric constants. Lastly, the cytotoxicity in murine tumor cell suspensions was investigated for the novel porphyrin-fullerene complex by inducing photodynamic treatments and determining the associated cell survival.

Paper Details

Date Published: 11 February 2008
PDF: 8 pages
Proc. SPIE 6845, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVII, 68450T (11 February 2008); doi: 10.1117/12.763461
Show Author Affiliations
O. Mermut, INO, Biophotonics (Canada)
McMaster Univ. (Canada)
J.-P. Bouchard, INO, Biophotonics (Canada)
J.-F. Cormier, INO, Biophotonics (Canada)
P. Desroches, INO, Biophotonics (Canada)
K. R. Diamond, McMaster Univ. (Canada)
M. Fortin, INO, Biophotonics (Canada)
P. Gallant, INO, Biophotonics (Canada)
S. Leclair, INO, Biophotonics (Canada)
J.-S. Marois, Univ. Laval (Canada)
I. Noiseux, INO, Biophotonics (Canada)
J.-F. Morin, Univ. Laval (Canada)
M. S. Patterson, McMaster Univ. (Canada)
M. Vernon, INO, Biophotonics (Canada)

Published in SPIE Proceedings Vol. 6845:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVII
David Kessel, Editor(s)

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