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Proceedings Paper

Combined use of fluorescent and dynamic light scattering imaging for applications in vascular biology
Author(s): Vyacheslav Kalchenko M.D.; Keren Ziv; Yoseph Addadi; Michal Neeman; Alon Harmelin D.V.M.
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Paper Abstract

We present an application of custom-designed Dynamic Light Scattering Imager (DLSI) in combination with conventional fluorescence intravital microscope (FIVM). The proposed technology was used for simultaneous examination of blood and lymphatic vessels in the mouse ear or tumor development. DLSI comprised a 650 nm diode laser with beam expander. Temporal fluctuations of laser interference pattern were used for rendering blood vessels anatomy and perfusion with a high spatial resolution. Concomitantly, various fluorescent contrast materials were used for labeling and visualization of lymphatic vessels or the tumor cells. The modular design of FIVM-DLSI allowed easy switching between different models of microscopes while conventional image sensors could be employed for both fluorescence and DLS imaging. We demonstrated that coupling with DLSI expands the fluorescent microscope imaging capabilities and does not compromise its ability to image with a high spatial resolution.

Paper Details

Date Published: 6 February 2008
PDF: 8 pages
Proc. SPIE 6855, Complex Dynamics and Fluctuations in Biomedical Photonics V, 68550D (6 February 2008); doi: 10.1117/12.763457
Show Author Affiliations
Vyacheslav Kalchenko M.D., Weizmann Institute of Science (Israel)
Keren Ziv, Weizmann Institute of Science (Israel)
Yoseph Addadi, Weizmann Institute of Science (Israel)
Michal Neeman, Weizmann Institute of Science (Israel)
Alon Harmelin D.V.M., Weizmann Institute of Science (Israel)

Published in SPIE Proceedings Vol. 6855:
Complex Dynamics and Fluctuations in Biomedical Photonics V
Valery V. Tuchin; Lihong V. Wang, Editor(s)

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