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Proceedings Paper

Monitoring the effect of mechanical stress on mesenchymal stem cell collagen production by multiphoton microscopy
Author(s): Wei-Liang Chen; Chia-Cheng Chang; Ling-Ling Chiou; Tsung-Hsien Li; Yuan Liu; Hsuan-Shu Lee M.D.; Chen-Yuan Dong
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Paper Abstract

Tissue engineering is emerging as a promising method for repairing damaged tissues. Due to cartilage's common wear and injury, in vitro production of cartilage replacements have been an active area of research. Finding the optimal condition for the generation of the collagen matrix is crucial in reproducing cartilages that closely match those found in human. Using multiphoton autofluorescence and second-harmonic generation (SHG) microscopy we monitored the effect of mechanical stress on mesenchymal stem cell collagen production. Bone marrow mesenchymal stem cells in the form of pellets were cultured and periodically placed under different mechanical stress by centrifugation over a period of four weeks. The differently stressed samples were imaged several times during the four week period, and the collagen production under different mechanical stress is characterized.

Paper Details

Date Published: 6 February 2008
PDF: 8 pages
Proc. SPIE 6858, Optics in Tissue Engineering and Regenerative Medicine II, 685806 (6 February 2008); doi: 10.1117/12.763227
Show Author Affiliations
Wei-Liang Chen, National Taiwan Univ. (Taiwan)
Chia-Cheng Chang, National Taiwan Univ. (Taiwan)
Ling-Ling Chiou, National Taiwan Univ. (Taiwan)
Tsung-Hsien Li, National Taiwan Univ. (Taiwan)
Yuan Liu, National Taiwan Univ. (Taiwan)
Hsuan-Shu Lee M.D., National Taiwan Univ. (Taiwan)
National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ. College of Medicine (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6858:
Optics in Tissue Engineering and Regenerative Medicine II
Sean J. Kirkpatrick; Ruikang K. Wang, Editor(s)

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