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Proceedings Paper

Label-free in vivo imaging of Drosophila melanogaster by multiphoton microscopy
Author(s): Chiao-Ying Lin; Vladimir Hovhannisyan; June-Tai Wu; Sung-Jan Lin; Chii-Wann Lin; Jyh-Horng Chen; Chen-Yuan Dong
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Paper Abstract

The fruit fly Drosophila melanogaster is one of the most valuable organisms in genetic and developmental biology studies. Drosophila is a small organism with a short life cycle, and is inexpensive and easy to maintain. The entire genome of Drosophila has recently been sequenced (cite the reference). These advantages make fruit fly an attractive model organism for biomedical researches. Unlike humans, Drosophila can be subjected to genetic manipulation with relative ease. Originally, Drosophila was mostly used in classical genetics studies. In the model era of molecular biology, the fruit fly has become a model organ for developmental biology researches. In the past, numerous molecularly modified mutants with well defined genetic defects affecting different aspects of the developmental processes have been identified and studied. However, traditionally, the developmental defects of the mutant flies are mostly examined in isolated fixed tissues which preclude the observation of the dynamic interaction of the different cell types and the extracellular matrix. Therefore, the ability to image different organelles of the fruit fly without extrinsic labeling is invaluable for Drosophila biology. In this work, we successfully acquire in vivo images of both developing muscles and axons of motor neurons in the three larval stages by using the minimially invasive imaging modality of multiphoton (SHG) microscopy. We found that while SHG imaging is useful in revealing the muscular architecture of the developing larva, it is the autofluorescence signal that allows label-free imaging of various organelles to be achieved. Our results demonstrate that multiphoton imaging is a powerful technique for investigation the development of Drosophila.

Paper Details

Date Published: 15 February 2008
PDF: 7 pages
Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 686019 (15 February 2008); doi: 10.1117/12.763202
Show Author Affiliations
Chiao-Ying Lin, National Taiwan Univ. (Taiwan)
Vladimir Hovhannisyan, National Taiwan Univ. (Taiwan)
June-Tai Wu, National Taiwan Univ. Hospital (Taiwan)
Sung-Jan Lin, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ. (Taiwan)
Chii-Wann Lin, National Taiwan Univ. (Taiwan)
Jyh-Horng Chen, National Taiwan Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6860:
Multiphoton Microscopy in the Biomedical Sciences VIII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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