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Proceedings Paper

Two-photon imaging using a flexible endoscope
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Paper Abstract

Two-photon autofluorescence imaging offers the analysis of cells and tissues without the need of taking biopsies, staining and complicated confocal detection systems. Therefore, it is of special interest for non- or minimal invasive clinical diagnostics. Until now, two-photon imaging was performed only on superficial surfaces like skin or of biopsies. To extend this technique to deeper tissues or inside the body the optical properties have to be reduced to endoscopical sizes. This can be achieved by tiny GRIN-optics, based on a radial gradient in the reflective index. A newly developed GRIN-lens assembly with increased numerical aperture is of special interest which is shown by the quality of tissue constituents and cell autofluorescence images. A fiber directs the laser light to the specimen in an assembly like an endoscope. This well-characterized photonic crystal fiber supports the high laser power of the femtosecond excitation impulses without the generation of non-linearities. A sensitive PMT detector detects the fluorescence. First fluorescence images using a fiber-GRIN lens combination were taken.

Paper Details

Date Published: 21 February 2008
PDF: 8 pages
Proc. SPIE 6851, Endoscopic Microscopy III, 68510B (21 February 2008); doi: 10.1117/12.762970
Show Author Affiliations
Iris Riemann, Fraunhofer Institute of Biomedical Technology (Germany)
Selma Schenkl, Fraunhofer Institute of Biomedical Technology (Germany)
Saarland Univ. (Germany)
Ronan Le Harzic, Fraunhofer Institute of Biomedical Technology (Germany)
JenLab GmbH (Germany)
Daniel Sauer, Fraunhofer Institute of Biomedical Technology (Germany)
Alexander Ehlers, Fraunhofer Institute of Biomedical Technology (Germany)
Saarland Univ. (Germany)
Bernhard Messerschmidt, Grintech GmbH (Germany)
Rainer Bückle, JenLab GmbH (Germany)
Karsten König, Saarland Univ. (Germany)
JenLab GmbH (Germany)

Published in SPIE Proceedings Vol. 6851:
Endoscopic Microscopy III
Guillermo J. Tearney; Thomas D. Wang, Editor(s)

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