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Proceedings Paper

Two-photon bioimaging with a multi-kilowatt peak-power optical pulse source using a 1-micron wavelength mode-locked semiconductor laser
Author(s): Hengchang Guo; Keijiro Takashima; Aya Sato; Hiroyuki Yokoyama; Masahito Mure; Yuji Iseki; Hiroshi Tsubokawa
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Paper Abstract

We have developed a multi-kilowatt peak power 1-μm optical pulse source for two-photon microscopy. Utilizing an external-cavity hybrid mode-locked semiconductor laser, we were able to generate picosecond optical pulses at a 500-MHz repetition rate. With a semiconductor optical amplifier driven by synchronized electronic gating pulses, the optical pulse repetition rate was sub-harmonically extracted at 1-100 MHz. At a 10-MHz repetition rate, optical pulses were then amplified to a peak power of greater than 2 kW with a two-stage Yb-doped fiber amplifier. Using this light source, we successfully obtained clear two-photon images of mouse brain neurons expressing green fluorescent proteins.

Paper Details

Date Published: 15 February 2008
PDF: 8 pages
Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 686020 (15 February 2008); doi: 10.1117/12.762922
Show Author Affiliations
Hengchang Guo, Tohoku Univ. (Japan)
Keijiro Takashima, Tohoku Univ. (Japan)
Aya Sato, Tohoku Univ. (Japan)
Hiroyuki Yokoyama, Tohoku Univ. (Japan)
Masahito Mure, Sumitomo Osaka Cement Co., Ltd. (Japan)
Yuji Iseki, Sumitomo Osaka Cement Co., Ltd. (Japan)
Hiroshi Tsubokawa, Tohoku Univ. (Japan)

Published in SPIE Proceedings Vol. 6860:
Multiphoton Microscopy in the Biomedical Sciences VIII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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