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Proceedings Paper

Dissolved oxygen sensing using organometallic dyes deposited within a microfluidic environment
Author(s): Q. L. Chen; H. P. Ho; L. Jin; B. W.-K. Chu; M. J. Li; V. W.-W. Yam
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Paper Abstract

This work primarily aims to integrate dissolved oxygen sensing capability with a microfluidic platform containing arrays of micro bio-reactors or bio-activity indicators. The measurement of oxygen concentration is of significance for a variety of bio-related applications such as cell culture and gene expression. Optical oxygen sensors based on luminescence quenching are gaining much interest in light of their low power consumption, quick response and high analyte sensitivity in comparison to similar oxygen sensing devices. In our microfluidic oxygen sensor device, a thin layer of oxygen-sensitive luminescent organometallic dye is covalently bonded to a glass slide. Micro flow channels are formed on the glass slide using patterned PDMS (Polydimethylsiloxane). Dissolved oxygen sensing is then performed by directing an optical excitation probe beam to the area of interest within the microfluidic channel. The covalent bonding approach for sensor layer formation offers many distinct advantages over the physical entrapment method including minimizing dye leaching, ensuring good stability and fabrication simplicity. Experimental results confirm the feasibility of the device.

Paper Details

Date Published: 13 February 2008
PDF: 8 pages
Proc. SPIE 6886, Microfluidics, BioMEMS, and Medical Microsystems VI, 68860O (13 February 2008); doi: 10.1117/12.762666
Show Author Affiliations
Q. L. Chen, The Chinese Univ. of Hong Kong (Hong Kong China)
H. P. Ho, The Chinese Univ. of Hong Kong (Hong Kong China)
L. Jin, The Chinese Univ. of Hong Kong (Hong Kong China)
B. W.-K. Chu, The Univ. of Hong Kong (Hong Kong China)
M. J. Li, The Univ. of Hong Kong (Hong Kong China)
V. W.-W. Yam, The Univ. of Hong Kong (Hong Kong China)

Published in SPIE Proceedings Vol. 6886:
Microfluidics, BioMEMS, and Medical Microsystems VI
Wanjun Wang; Claude Vauchier, Editor(s)

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