Injection and delivery of small amount reagent in aqueous solution for cell chip was performed utilizing regeneratively amplified femtosecond laser system. In our new trial, the reagent integrated on a solid strip are released and delivered to targeted cells with the femutosecond laser-induced impulsive-force. The reagent was fixed in poly(vinyl alcohol) or polystyrene film on a glass-substrate strip. When a single pulsed femtosecond laser was focused in the solution, the film near the focal point was fragmented and the reagent was dispersed in 45-μm φ area at 50 μm from the surface of the reagent strip. As examples cardiomyocyte beating cells of P19CL6 were bombed with epinephrine and acetylcholine, and as a result the beating ratio of the cells were quickly stimulated and suppressed, respectively. The results demonstrate that the present method is a promising key nano/micro technology for diagnosis and drug discovery.

Date Published: 25 February 2008
PDF: 7 pages
Proc. SPIE 6854, Optical Interactions with Tissue and Cells XIX, 685414 (25 February 2008); doi: 10.1117/12.762613

Published in SPIE Proceedings Vol. 6854:
Optical Interactions with Tissue and Cells XIX
Steven L. Jacques; William P. Roach; Robert J. Thomas, Editor(s)