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Proceedings Paper

Monitoring circulating apoptotic cells by in-vivo flow cytometry
Author(s): Xunbin Wei; Yuan Tan; Yun Chen; Li Zhang; Yan Li; Guangda Liu; Bin Wu; Chen Wang
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Paper Abstract

Chemotherapies currently constitute one main venue of cancer treatment. For a large number of adult and elderly patients, however, treatment options are poor. These patients may suffer from disease that is resistant to conventional chemotherapy or may not be candidates for curative therapies because of advanced age or poor medical conditions. To control disease in these patients, new therapies must be developed that are selectively targeted to unique characteristics of tumor cell growth and metastasis. A reliable early evaluation and prediction of response to the chemotherapy is critical to its success. Chemotherapies induce apoptosis in tumor cells and a portion of such apoptotic cancer cells may be present in the circulation. However, the fate of circulating tumor cells is difficult to assess with conventional methods that require blood sampling. We report the in situ measurement of circulating apoptotic cells in live animals using in vivo flow cytometry, a novel method that enables real-time detection and quantification of circulating cells without blood extraction. Apoptotic cells are rapidly cleared from the circulation with a half-life of ~10 minutes. Real-time monitoring of circulating apoptotic cells can be useful for detecting early changes in disease processes, as well as for monitoring response to therapeutic intervention.

Paper Details

Date Published: 18 February 2008
PDF: 8 pages
Proc. SPIE 6857, Biophotonics and Immune Responses III, 68570A (18 February 2008); doi: 10.1117/12.762574
Show Author Affiliations
Xunbin Wei, Fudan Univ. (China)
Yuan Tan, Fudan Univ. (China)
Yun Chen, Fudan Univ. (China)
Li Zhang, Fudan Univ. (China)
Yan Li, Fudan Univ. (China)
Guangda Liu, Fudan Univ. (China)
Bin Wu, Phosphorex, Inc. (United States)
Chen Wang, College of Medical Apparatus and Instruments, The Univ. of Shanghai for Science and Technology (China)

Published in SPIE Proceedings Vol. 6857:
Biophotonics and Immune Responses III
Wei R. Chen, Editor(s)

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