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Proceedings Paper

Structural and biochemical characterization of engineered tissue using FTIR spectroscopic imaging: melanoma progression as an example
Author(s): Rohit Bhargava; Rong Kong
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Paper Abstract

Engineered tissue represents a convenient path to providing models for imaging and disease progression. The use of these models or phantoms is becoming increasingly prevalent. While structural characterization of these systems is well-documented, a combination of biochemical and structural knowledge is often helpful. Fourier transform infrared (FTIR) spectroscopic imaging is a rapidly emerging technique that combines the molecular selectivity of spectroscopy with the spatial specificity of optical microscopy. Here, we report on the application of FTIR spectroscopic for analysis of a melanoma model in engineered skin. We first characterize the biochemical properties, consistency and spectral changes in different layers of growing skin. Results provide simple indices for monitoring tissue consistency and reproducibility as a function of time. Second, we introduce malignant melanocytes to simulate tumor formation and growth. Both cellular changes associated with tumor formation and growth can be observed. FTIR images indicate holistic chemical changes during the tumor growth, allowing for the development of automated pathology protocols. FTIR imaging being non-destructive, further, samples remain entirely compatible with downstream tissue processing or staining. We specifically examined the correlation of structural changes, molecular content and reproducibility of the model systems. The development of analysis, integrating spectroscopy, imaging and computation will allow for quality control and standardization of both the structural and biochemical properties of tissue phantoms.

Paper Details

Date Published: 21 February 2008
PDF: 10 pages
Proc. SPIE 6870, Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue, 687004 (21 February 2008); doi: 10.1117/12.762511
Show Author Affiliations
Rohit Bhargava, Univ. of Illinois at Urbana-Champaign (United States)
Rong Kong, Univ. of Illinois at Urbana-Champaign (United States)

Published in SPIE Proceedings Vol. 6870:
Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue
Robert J. Nordstrom, Editor(s)

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