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Proceedings Paper

Nano-aquarium for dynamic observation of aquatic microorganisms fabricated by femtosecond laser direct writing of photostructurable glass
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Paper Abstract

We demonstrate the fabrication of three-dimensional (3-D) hollow microstructures embedded in photostructurable glass by a femtosecond (fs) laser direct writing. Fs laser direct writing followed by annealing and successive wet etching in dilute hydrofluoric (HF) acid solution resulted in the rapid manufacturing of microchips with 3-D hollow microstructures for the dynamic observation of living microorganisms in fresh water. The embedded microchannel structure enables us to analyze the continuous motion of Euglena gracilis. A microchamber with a movable microneedle demonstrates its ability for the elucidation of the information transmission process in Pleurosira laevis. Such microchips, referred to as nano-aquariums realize the efficient and highly functional observation of microorganisms.

Paper Details

Date Published: 23 February 2008
PDF: 9 pages
Proc. SPIE 6880, Laser-based Micro- and Nanopackaging and Assembly II, 68800E (23 February 2008); doi: 10.1117/12.762391
Show Author Affiliations
Y. Hanada, RIKEN - The Institute of Physical and Chemical Research (Japan)
K. Sugioka, RIKEN - The Institute of Physical and Chemical Research (Japan)
H. Kawano, RIKEN - Brain Science Institute (Japan)
I. Ishikawa, RIKEN - Brain Science Institute (Japan)
A. Miyawaki M.D., RIKEN - Brain Science Institute (Japan)
K. Midorikawa, RIKEN - The Institute of Physical and Chemical Research (Japan)

Published in SPIE Proceedings Vol. 6880:
Laser-based Micro- and Nanopackaging and Assembly II
Wilhelm Pfleging; Yongfeng Lu; Kunihiko Washio; Willem Hoving; Jun Amako, Editor(s)

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