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Proceedings Paper

Phenotypic analysis of bacterial colonies using laser light scatter and pattern-recognition techniques
Author(s): Bartek Rajwa; Bulent Bayraktar; Padmapriya P. Banada; Karleigh Huff; Euiwon Bae; E. Daniel Hirleman; Arun K. Bhunia; J. Paul Robinson
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Paper Abstract

The formation of bacterial colonies and biofilms requires coordinated gene expression, regulated cell differentiation, autoaggregation, and intercellular communication. Therefore colonies of bacteria have been recognized as multicellular organisms or "superorganisms." It has consequently been postulated that the phenotype of colonies formed by microorganisms can be automatically recognized and classified using optical systems capable of collecting information related to cellular pattern formation and morphology of colonies. Recently we have reported a first practical implementation of such a system, capable of noninvasive, label-free classification and recognition of pathogenic Listeria species. The design employed computer-vision and pattern-recognition techniques to classify scatter patterns produced by bacterial colonies irradiated with laser light. Herein we report our efforts to extend this system to other genera of bacteria such as Salmonella, Vibrio, Staphylococcus, and E. coli. Application of orthogonal moments, as well as texture descriptors for image feature extraction, provides high robustness in the presence of noise. An improved pattern classification scheme based on an SVM algorithm provides better results than the previously employed neural network system. Low error rates determined by cross-validation, reproducibility of the measurements, and overall robustness of the recognition system prove that the proposed technology can be implemented in automated devices for bacterial detection.

Paper Details

Date Published: 22 February 2008
PDF: 7 pages
Proc. SPIE 6864, Biomedical Applications of Light Scattering II, 68640S (22 February 2008); doi: 10.1117/12.762366
Show Author Affiliations
Bartek Rajwa, Purdue Univ. (United States)
Bulent Bayraktar, Purdue Univ. (United States)
Padmapriya P. Banada, Purdue Univ. (United States)
Karleigh Huff, Purdue Univ. (United States)
Euiwon Bae, Purdue Univ. (United States)
E. Daniel Hirleman, Purdue Univ. (United States)
Arun K. Bhunia, Purdue Univ. (United States)
J. Paul Robinson, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 6864:
Biomedical Applications of Light Scattering II
Adam Wax; Vadim Backman, Editor(s)

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