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Proceedings Paper

Autofluorescence dynamics during reperfusion following long-term renal ischemia in a rat model
Author(s): Rajesh N. Raman; Christopher D. Pivetti; Dennis L. Matthews; Christoph Troppmann; Stavros G. Demos
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Paper Abstract

Optical properties of near-surface kidney tissue were monitored in order to assess response during reperfusion to long (20 minutes) versus prolonged (150 minutes) ischemia in an in vivo rat model. Specifically, autofluorescence images of the exposed surfaces of both the normal and the ischemic kidneys were acquired during both injury and reperfusion alternately under 355 nm and 266 nm excitations. The temporal profile of the emission of the injured kidney during the reperfusion phase under 355 nm excitation was normalized to that under 266 nm as a means to account for changes in tissue optical properties independent of ischemia as well as changes in the illumination/collection geometrical parameters in future clinical implementation of this technique using a hand-held probe. The scattered excitation light signal was also evaluated as a reference signal and found to be inadequate. Characteristic time constants were extracted using a fit to a relaxation model and found to have larger mean values following 150 minutes of injury. The mean values were then compared with the outcome of a chronic survival study where the control kidney had been removed. Rat kidneys exhibiting longer time constants were much more likely to fail. This may lead to a method to assess kidney viability and predict its ability to recover in the initial period following transplantation or resuscitation.

Paper Details

Date Published: 20 February 2008
PDF: 8 pages
Proc. SPIE 6853, Biomedical Optical Spectroscopy, 685318 (20 February 2008); doi: 10.1117/12.762363
Show Author Affiliations
Rajesh N. Raman, Univ. of California, Davis (United States)
Christopher D. Pivetti, Univ. of California, Davis Medical Ctr. (United States)
Dennis L. Matthews, Univ. of California, Davis (United States)
Christoph Troppmann, Univ. of California, Davis Medical Ctr. (United States)
Stavros G. Demos, Lawrence Livermore National Lab. (United States)
Univ. of California, Davis Medical Ctr. (United States)

Published in SPIE Proceedings Vol. 6853:
Biomedical Optical Spectroscopy
Anita Mahadevan-Jansen; Robert R. Alfano; Alvin Katz; Wolfgang Petrich, Editor(s)

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